Amoeba Sisters
Amoeba SistersExplore an intro to genetic engineering with The Amoeba Sisters. This video provides a general definition, introduces some biotechnology tools that can be used in genetic engineering, and discusses some related vocabulary (such as plasmid, vector, transformation, transgenic, and more). CRISPR is also mentioned; expand to see further reading to explore more. To see more Amoeba Sisters biotechnology related videos, visit https://www.youtube.com/playlist?list=PLwL0Myd7Dk1Gxs9mwISTCEi8i1xex94p2
Table of Contents:
00:00 Intro
1:51 Genetic Engineering Defined
2:01 Insulin Production in Bacteria
3:55 Some Vocab
4:20 Vectors & More
5:25 CRISPR
6:40 Genetic Engineering Uses
7:30 Ethics
Factual References:
Clark, M. A., Douglas, M., & Choi, J. (2018, March 18). 17.1 Biotechnology – Biology 2e | OpenStax. Openstax.org. https://openstax.org/books/biology-2e/pages/17-1-biotechnology
Urry, Lisa A, et al. Campbell Biology. 11th ed., New York, Ny, Pearson Education, Inc, 2017.
Zedalis, J., & Eggebrecht, J. (2018, March 8). 17.1 Biotechnology – Biology for AP® Courses | OpenStax. Openstax.org. https://openstax.org/books/biology-ap-courses/pages/17-1-biotechnology
FURTHER READING:
Note: our description is limited in characters; we encourage you to keep exploring beyond these recommendations!
*GFP and fluorescence?
https://embryo.asu.edu/pages/green-fluorescent-protein
*This link has a great bacterial transformation exploration!
*How is genetic engineering (which video focuses on) & gene therapy (which video doesn’t mention) DIFFERENT? — this also talks about using viruses:
https://medicine.missouri.edu/centers-institutes-labs/health-ethics/faq/gene-therapy
*Includes charts of different cells used to produce biopharmaceuticals:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4748523/
*History of synthesizing growth factor (interesting!)
https://americanhistory.si.edu/blog/2012/10/human-growth-hormone.html
*Genetically engineered plants and…
**Pollutants in air or soils:
https://www.nih.gov/news-events/nih-research-matters/trees-created-clean-pollutants
https://www.nih.gov/news-events/nih-research-matters/genetically-modified-houseplant-cleans-indoor-air#
https://onlinelibrary.wiley.com/doi/10.1111/pce.12963
**Climate change mitigation?
https://doi.org/10.1016/j.tplants.2022.01.004
*Genetically Engineered Chickens and Avian Influenza Resistance?https://doi.org/10.3390/v10100561https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6149294/
*Genetically Engineered Mice:
https://www.ncbi.nlm.nih.gov/books/NBK231336/
*More on CRISPR:
https://medlineplus.gov/genetics/understanding/genomicresearch/genomeediting/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971521/
**Book recommendation:
“The Code Breaker: Jennifer Doudna, Gene Editing, and the Future of the Human Race” by Walter Isaacson
ETHICAL ISSUES:
*What is bioethics? (from Michigan State University)
https://bioethics.msu.edu/what-is-bioethics
*CRISPR and Gene Editing Ethical Issues:
https://doi.org/10.21775/cimb.026.103
https://www.genome.gov/about-genomics/policy-issues/Genome-Editing/ethical concerns
*Agriculture:
https://www.nature.com/scitable/topicpage/genetically-modified-organisms-gmos-transgenic-crops-and-732/
*Animals:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3078015/#genetics
GENETIC ENGINEERING AS A CAREER:
The US Bureau of Labor Statistics puts genetic engineering in the category of bioengineers: https://www.bls.gov/ooh/architecture-and-engineering/biomedical-engineers.htm
The Amoeba Sisters videos demystify science with humor and relevance. The videos center on Pinky’s certification and experience in teaching biology at the high school level. Amoeba Sisters videos only cover concepts that Pinky is certified to teach, and they focus on her specialty: secondary life science. Learn more about our videos here: https://www.amoebasisters.com/our-videos
Support Us? https://www.amoebasisters.com/support-us
Our Resources and Handouts: https://www.amoebasisters.com/handouts
Biology Video Playlist: https://www.youtube.com/playlist?list=PLwL0Myd7Dk1F0iQPGrjehze3eDpco1eVz
MUSIC:
Our intro music designed and performed by Jeremiah Cheshire.
End music in this video is listed free to use/no attribution required from the YouTube audio library.
TRANSLATIONS:
Indonesian Subtitles Credit: Hanifah Yumna
We gladly accept subtitle translations from our community. Learn more here: https://www.amoebasisters.com/pinkys-ed-tech-favorites/community-contributed-subtitles
We also have videos using an artificial voice via https://aloud.area120.google.com to increase accessibility. See our dubbed channels on https://www.amoebasisters.com/pinkys-ed-tech-favorites/community-contributed-subtitles
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35 Comments
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02/13/2025 - 9:01 AMCan you make a video about Geological Timescale??
@liaselleaeri
02/13/2025 - 9:01 AMi understood nothing 🙁
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02/13/2025 - 9:01 AMKeep these videos coming, you've helped a lot of people through bio in IGCSE and A levels
@aqeebhussain232
02/13/2025 - 9:01 AMHi. I am a first year medical student and have molecular biology as a subject, and I was wondering, where does restriction enzymes occur? I have heard that they occur in bacteria and other prokaryotic organisms, but if I have mistaken please help me to get the right answer.
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02/13/2025 - 9:01 AMAnyone have Mrs. Beecham?
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02/13/2025 - 9:01 AMGoes to an Ivy league school – but is 100% taught and carried by the amoeba sisters.
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02/13/2025 - 9:01 AMMy favourite channel ❤
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02/13/2025 - 9:01 AMAmeoba sisters out here saving my life.
– An evolutation and ecology major
@manaljarrar
02/13/2025 - 9:01 AMشرح Genetic Engineering بالعربي..
@AbdalkaderAl-khatib
02/13/2025 - 9:01 AMThank you 🙏🏻
@mariyamkhan5138
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@WoapyisDopey
02/13/2025 - 9:01 AM@ 3:28 BE NICE TO HIM ! 🥺
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02/13/2025 - 9:01 AMControl+x control+v 2:30
@jameselroy7472
02/13/2025 - 9:01 AMType 1 diabetes does not produce insulin but type 2 makes a small amount of insulin. This is why type 1 can go into DKA and type 2 does not. Type 2 goes through HHS because they dont utilize the ketone pathway due to the liver constantly attempting to deliver glucose to the cells. Basically the liver is confused because the body still makes insulin. This is why type 2 diabetes can have higher 900db blood sugars for longer time and be more dehydrated before hosiptalization unlike type 1 where their blood sugar will be more like 600 db with massive ketone elevation at a faster rate. The treatment is the same due to electrolyte and ion shifts. Basically watch and replace potassium PRN, give fluids and iv insulin. Obviously this is an over simplified statement.
@GOJOSATORU-o5o
02/13/2025 - 9:01 AMvery good information
@noworneversoulbeach
02/13/2025 - 9:01 AMStarted off the video with “Terra Formars” abilities…👀
@jingqiz
02/13/2025 - 9:01 AMOMG MY SAVIOUR
@H4CK41D
02/13/2025 - 9:01 AMWhat highschool did you go to?! PCR test genetics lab and now this!
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02/13/2025 - 9:01 AMI stumbled upon Hidden DNA Potential on Shirlest, and let me tell you, it opened my eyes to possibilities I never knew existed. A must-read for anyone wanting to elevate their life!
@etsap1236
02/13/2025 - 9:01 AMGenitive engineering is definitely one of those sciences that I think needs to have an eye kept on it. Use it for insulin or some medical conditions, not on people. This is coming from someone with a genetic disease. I would rather be the way I am now, then chosen to be what I could of been by others. Because even though the epilepsy is horrible I’d rather treat it than have lived my whole life without it. If I never had it I would probably be a pretty different person. I cant drive and my memory is shit, but that’s shaped many things about me. I’m just me.
@HadichaSolih-y9i
02/13/2025 - 9:01 AMThank you for creating such an useful and informative video i was struggling so much to understand what was the genetic engineering ❤️❤️❤️❤️❤️🫶🫶🫶🫶🫶🫶
@jessicasibilla9256
02/13/2025 - 9:01 AMdo you guys have a video in insulin production?
@kishengounden1891
02/13/2025 - 9:01 AMI have a question,do they used genetic engineering on humans like people with mental illness?
@twoocake
02/13/2025 - 9:01 AMthanks so muchhh!!!!
@aaronsaunders6974
02/13/2025 - 9:01 AMNeat 😊
@ExamdecoderAa
02/13/2025 - 9:01 AMThanks amoeba sister ❤
@gutkhanewton5499
02/13/2025 - 9:01 AMGraphics are funny , learning from u is fun 😊
@TejasSoma
02/13/2025 - 9:01 AMmy father has diabites
@yangtzemian1240
02/13/2025 - 9:01 AMThis channel is the best🤩thank you so much Amoeba Sisters!💗
@Spacekid_Productions
02/13/2025 - 9:01 AMsaving my GCSE fr